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Description
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This database includes the alerts reported by the RASFF system, the UHPLC-MS parameters used in the study,the results obtained for the optimization of the uSPEed(r) extraction procedure and the validation of this methodology as well as the results of matrix effect and recovery percentages. Also includes the AGREEprep scores obtained for the methodology. Finally, the concentrations of PAs and TAs found in the infusion of dry edible flowers using the proposed analytical methodology (2024-06-01)
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Notes
| A miniaturized solid-phase extraction of two tropane alkaloids (TAs) and twenty-one pyrroliz-idine alkaloids (PAs) from infusions of dry edible flowers using optimized µSPEed® technique was developed. The optimization of the µSPEed® methodology involved testing different car-tridges and comparing various volumes and numbers of loading cycles. The final conditions al-lowed for a rapid extraction, taking only 3.5 minutes. This was achieved using a C18-ODS car-tridge, conditioning with 100 µL of methanol (2 cycles), loading 100 µL of the infusion sample (7 cycles), and eluting the analytes with 100 µL of methanol (2 cycles). Prior to their analysis by UHPLC-IT-MS/MS, the extracts were evaporated and reconstituted in 100 µL of water (0.2 % formic acid)/methanol (0.2 % ammonia) 95:5 (v/v), allowing for a preconcentration factor of 7 times. The methodology was successfully validated obtaining recoveries ranging between 87 and 97 %, RSD of less than 12 %, and MQL between 0.09 and 0.2 µg/L. The validated methodology was applied to twenty samples of edible flower infusions to evaluate the safety of these products. Two infusion samples obtained from Acmella oleracea and Viola tricolor were contaminated with 0.16 and 0.2 µg/L of scopolamine (TA) respectively, while the infusion of Citrus aurantium was contami-nated with intermedine and lycopsamine (PAs) below the MQL |
