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Notas
| Methodology: Whole-transcriptome analysis was done on RNA samples with Illumina total RNA-Seq technology using the ScriptSeq Complete kit (Illumina RS-122-2201), which entailed rRNA removal, cDNA synthesis, 3´ terminal tagging, and PCR purification, followed by sequencing using the NextSeq 550 Sequencing kit and system (Illumina). Reads were generated from raw total RNA-Seq data and mapped to the human genome, and the htseq-count tool was used to count the number of reads mapping each gene. Differential expression analysis was then performed on raw total RNA-Seq data using the DESeq2 package |
