Differential gene expression of neural stem cells in proliferation and dopaminergic differentiation conditions

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Nelke, Anna; Garcia Lopez, Silvia; Martinez Serrano, Alberto; Pereira, Marta, 2021, "Differential gene expression of neural stem cells in proliferation and dopaminergic differentiation conditions", https://doi.org/10.21950/4IXBTX, e-cienciaDatos, V1
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Description

This NGS study was done in order to analyze the differential gene expression of neural stem cells during proliferation and induction of differentiation in vitro. All samples are human neural stem cells derived from fetal ventral mesencephalon (hVM1 clone 32 cell line). Experimental conditions included cells in proliferation and cells induced for dopaminergic differentiation.

Dataset: The dataset includes 4 files: two sets of differentially expressed genes (with and without null values) and two sets of expression data with null values (for both proliferating and differentiated cells).

Subject Medicine, Health and Life Sciences
Keyword NGS, transcriptomics, stem cells
Related Publication Multifactoriality of Parkinson´s disease as explored through human neural stem cells and their transplantation in middle-aged Parkinsonian mice. Anna Nelke, Silvia García-López, Alberto Martínez-Serrano, Marta P Pereira
Notes Methodology: Whole-transcriptome analysis was done on RNA samples with Illumina total RNA-Seq technology using the ScriptSeq Complete kit (Illumina RS-122-2201), which entailed rRNA removal, cDNA synthesis, 3´ terminal tagging, and PCR purification, followed by sequencing using the NextSeq 550 Sequencing kit and system (Illumina). Reads were generated from raw total RNA-Seq data and mapped to the human genome, and the htseq-count tool was used to count the number of reads mapping each gene. Differential expression analysis was then performed on raw total RNA-Seq data using the DESeq2 package
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Proliferation_group_expression_data_with_null_values.xlsx
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Published Nov 25, 2021
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MD5: 8165b7f680cfbd95931802ba0503a643
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DESeq2_differentially_expressed_genes_with_null_values.xlsx
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Published Nov 25, 2021
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MD5: e4a1b9146a3337da4fe57d47814405e8
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DESeq2_differentially_expressed_genes.xlsx
MS Excel Spreadsheet - 1,6 MB
Published Nov 25, 2021
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MD5: 242f57376d1d2a6219a9308eaaae2771
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pereira_gene_readme_en.txt
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Published Nov 25, 2021
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MD5: b6c084fa0877d822ab9034b8575edb48
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Preview "pereira_gene_readme_en.txt"
Differentiation_group_expression_data_with_null_values.xlsx
MS Excel Spreadsheet - 2,6 MB
Published Nov 25, 2021
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MD5: 4c66428084c611573110816c097d7949
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