|
Descripción
|
Metagenomic study of environmental samples collected at a pig farm in Spain. This study is part of the HE-FARM project under the Horizon Europe programme, which aims to improve biosecurity on farms.
Bacterial genera present in several areas of a pig farm were analyzed using high-throughput sequencing techniques. Samples were collected from the skin and feces of animals, as well as from surfaces, water, and air. The analyses revealed a strong cor-relation between air and skin samples, supporting the idea that bacterial growth on skin is potentially a mechanism of aerosolization and airborne transport. A water–air trans-mission route also appears to be present, although the direction of the transport mecha-nism cannot yet be determined. Other potential routes, such as contact with surfaces or feces, seem to be less efficient.
This data contains the raw data used for metagenomic analyses (16S) of environmental samples in a pig farm.
It includes the genomic data for each sample analyzed and compared in the study titled 'Skin Aerosolisation Predominance in a Pig Farm'. The data is organized in a way that facilitates further exploration and analysis, ensuring that researchers can effectively utilize it for their own studies. (2023-09-11)
|
|
Publicación relacionada
| Pérez-Díaz, J.L.; del Álamo, C.; Aranguren- Rivas, P.; Peiró, S.; Muñoz, M.; Alcamí, A.; Vázquez-Calvo, Á.; Óvilo, C.; Calvo, L.; Morales, P.; et al. Skin Aerosolization Predominance in a Pig Farm. Aerobiology 2025 |
|
Notas
| A total of 67 samples were obtained from the farm, out of which sufficient DNA was extracted from 63 samples for 16S sequencing.The type of samples analyzed were environmental and animal samples: air, surfaces, water, animal skin and animal feces.
Sequencing libraries were generated using TruSeq® DNA PCR-Free Sample Preparation Kit (Illumina, USA), according to the manufacturer’s instructions, and index codes were added. Library quality was assessed using the Qubit@ 2.0 Fluorometer (Thermo Scientific) and the Agilent Bioanalyzer 2100 system. Finally, sequencing was performed on an Illumina NovaSeq platform, generating 250 bp paired-end reads. The sequencing of the 16S region was performed to assess the microbial composition of the collected samples. |
