Optimizing standardized lab-grown skin substitutes reveals a proliferation-differentiation switch based in ascorbic acid

Version 2.0
Molina-Oviedo, Angie Katherine; Sorrentino, Ilaria; Clares Pedrero, Irene; Salamanca-Gonzalez, Celina; Arévalo Núñez de Arenas, Eduardo; Mazariegos, Marina; CABANAS, CARLOS; Medraño Fernandez, Iria, 2025, "Optimizing standardized lab-grown skin substitutes reveals a proliferation-differentiation switch based in ascorbic acid", https://doi.org/10.21950/BUUT5Q, e-cienciaDatos, V2
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The project "From 2D to 3D: Optimization of lab-grown skin substitutes reveals a physiological switch based on ascorbic acid." aimed to provide culture conditioning and adjusting of keratinocytes intended to be used in differentiation processes. The keratinocytes were evaluated on their capacity to generate competent skin constructs as a model of cellular differentiation. The influence of ascorbic acid in 2D and 3D epidermal models was characterized, as it was found to act as a physiological switch.
This dataset comprises raw images obtained in the 2D and 3D analysis. The keratinocytes used correspond to HaCaT cells cultured under low calcium conditions. The results associated with FACS results should be carefully analyzed considering the differentiation trigger caused by detachment of the cells. For more information please refer to the related publications associated to this dataset.
Subject Medicine, Health and Life Sciences
Keyword Ascorbic acid, Keratinocytes, Organotypic constructs, Metabolite-induced switch, IntegrinB1
Related Publication Is Supplement To: Molina-Oviedo, A. K., Sorrentino, I., Clares-Pedrero, I., Salamanca-Gonzalez, C., Arevalo-Nuñez de Arenas, E., Mazariegos, M. S., Cabañas, C., & Medraño-Fernandez, I. (2025). Optimizing standardized lab-grown skin substitutes evidences a proliferation-differentiation switch based on ascorbic acid. iScience, 28(8), 113066. https://doi.org/10.1016/j.isci.2025.113066handle: 10016/47998
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Optimizing_standardized_lab-grown_2025_Readme.txt
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