Descripción
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This proteomic study was done in order to analyze the differential protein expression of neural stem cells during proliferation and induction of differentiation in vitro. All samples are human neural stem cells derived from fetal ventral mesencephalon (hVM1 clone 32 cell line). Experimental conditions included cells in proliferation and cells induced for dopaminergic differentiation.
Dataset: The dataset includes 5 files: a complete report on protein expression as well as functional analyses using the Qiagen Ingenuity Pathway Analysis including canonical pathways, diseases and disorders, biological functions, networks, and top upstream regulators, in proliferating and differentiated cells.
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Notas
| Methodology: Samples underwent high resolution label free quantitation liquid chromatography-electrospray ionization-tandem mass spectrometry, with the liquid chromatography system and mass spectrometer connected. Each sample underwent liquid chromatography using a C18 reversed phase column and eluted peptides were separated using a TripleTOF mass spectrometer (Sciex). Mass spectrometry data was analyzed employing four different database searches, namely Mascot Server version 2.5 (Matrix Science), OMSSA version 2.1.9 (NCBI), X! TANDEM version win-13-02-01-1 (The GPM), and Myrimatch version 2.1 (Vanderbilt University), against the Uniprot Homo sapiens database (78,120 proteins when last updated on 2021/03/07). |